Coding
RhaR

Part:BBa_K902069:Design

Designed by: Chris Wintersinger   Group: iGEM12_Calgary   (2012-10-02)

RhaR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 559
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was added to pSB1C3 using Biobrick Assembly Standards.

Source

PCR amplified from Top Ten E. coli using Kapa Hi-Fi polymerase.

References

Egan, S. M., Schleif, R. F., & others. (1993). A regulatory cascade in the induction of rhaBAD. Journal of Molecular Biology, 234(1), 87-98.